Journal: Medicine

Article Title: LEF1 is associated with immunosuppressive microenvironment of patients with lung adenocarcinoma

doi: 10.1097/md.0000000000039892

Figure Lengend Snippet: Figure 4. LEF1 is expressed in tumor cells and immune cells. (A) Immunofluorescence staining of LEF1 and Pan-CK in tumor tissues of LUAD patients. (B) Immunofluorescence staining of LEF1 and CD4 in tumor tissues of LUAD patients. (C) Immunofluorescence staining of LEF1 and CD68 in tumor tissues of LUAD patients. LUAD = lung adenocarcinoma.

Article Snippet: Prepared tissue sections as in immunohistochemical analysis, and then sections were incubated with LEF1 rabbit monoclonal antibody (1:100, ab137872, abcam), Pan-CK mouse monoclonal antibody (1:200, ab215838, abcam), APC conjugated CD4 mouse monoclonal antibody (82116, 1:2000, Cell Signaling Technology), CD68 mouse monoclonal antibody (1:500, 14-0688-82, Invitrogen), FITC conjugated CD68 mouse monoclonal antibody (1:100, MA1-82715, Invitrogen), CD163 mouse monoclonal antibody (1:500, ab156769) or FOXP3 rabbit monoclonal antibody (1:1000, ab215206, abcam), and incubated for 2 hours at room temperature.

Techniques: Immunofluorescence, Staining

Journal: Medicine

Article Title: LEF1 is associated with immunosuppressive microenvironment of patients with lung adenocarcinoma

doi: 10.1097/md.0000000000039892

Figure Lengend Snippet: Figure 5. Higher LEF1 expression is related to immunosuppressive microenvironment. (A) Correlation between LEF1 expression and B cell, CD8+ T cell, CD4+ T cell, macrophage, neutrophil, dendritic cell infiltration in the TIMER database. (B) Fraction of 21 tumor-infiltrating immune cells in low- and high-LEF1 expression group with data from TCGA database. (C) Heatmap of immunosuppressive molecules expression in low- and high-LEF1 expression group with data from TCGA database. Comparison between high- and low-LEF1 group, ***P < .001. TCGA = The Cancer Genome Atlas, TIMER = tumor immune estimation resource.

Article Snippet: Prepared tissue sections as in immunohistochemical analysis, and then sections were incubated with LEF1 rabbit monoclonal antibody (1:100, ab137872, abcam), Pan-CK mouse monoclonal antibody (1:200, ab215838, abcam), APC conjugated CD4 mouse monoclonal antibody (82116, 1:2000, Cell Signaling Technology), CD68 mouse monoclonal antibody (1:500, 14-0688-82, Invitrogen), FITC conjugated CD68 mouse monoclonal antibody (1:100, MA1-82715, Invitrogen), CD163 mouse monoclonal antibody (1:500, ab156769) or FOXP3 rabbit monoclonal antibody (1:1000, ab215206, abcam), and incubated for 2 hours at room temperature.

Techniques: Expressing, Comparison

Journal: Medicine

Article Title: LEF1 is associated with immunosuppressive microenvironment of patients with lung adenocarcinoma

doi: 10.1097/md.0000000000039892

Figure Lengend Snippet: Figure 7. Correlation between LEF1 expression and Treg cells. (A) Heatmap of Treg cells related mRNA (ENTPD1, FOXO1, FOXP3 and IL7R) expression in low- and high-LEF1 expression group with data from TCGA database. (B) Correlation between LEF1 expression and Treg cells related mRNA (ENTPD1, FOXO1, FOXP3 and IL7R) expression with data from TIMER database. (C) Immunohistochemical image of LEF1 (up), and immunofluorescence staining of LEF1 and CD4/FOXP3 in tumor tissues of LUAD patients (down). (D) Correlation between LEF1 expression and CD4+FOXP3+ cells in 105 cases tumor tissues of LUAD patients. Comparison between normal and tumor group, ***P < .001. LUAD = lung adenocarcinoma, TCGA = The Cancer Genome Atlas.

Article Snippet: Prepared tissue sections as in immunohistochemical analysis, and then sections were incubated with LEF1 rabbit monoclonal antibody (1:100, ab137872, abcam), Pan-CK mouse monoclonal antibody (1:200, ab215838, abcam), APC conjugated CD4 mouse monoclonal antibody (82116, 1:2000, Cell Signaling Technology), CD68 mouse monoclonal antibody (1:500, 14-0688-82, Invitrogen), FITC conjugated CD68 mouse monoclonal antibody (1:100, MA1-82715, Invitrogen), CD163 mouse monoclonal antibody (1:500, ab156769) or FOXP3 rabbit monoclonal antibody (1:1000, ab215206, abcam), and incubated for 2 hours at room temperature.

Techniques: Expressing, Immunohistochemical staining, Immunofluorescence, Staining, Comparison